Journal: AMB Express

Article Title: Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

doi: 10.1186/s13568-017-0381-6

Figure Lengend Snippet: ComX bioactivity in bacterial culture supernatants from late exponential phase B. licheniformis NCIMB 8874 and E. coli BL21 ectopically expressing a srfA - lacZ fusion, as determined through a β-galactosidase reporter ( B. subtilis JRL293). Data were collected from three independent experiments which were performed in triplicate. The constant blank (water) has been subtracted from all calculated values. The details of the tested culture supernatants are presented in the table

Article Snippet: Fig. 6 ComX bioactivity in bacterial culture supernatants from late exponential phase B. licheniformis NCIMB 8874 and E. coli BL21 ectopically expressing a srfA - lacZ fusion, as determined through a β-galactosidase reporter ( B. subtilis JRL293).

Techniques: Expressing

Journal: bioRxiv

Article Title: Expression of Pokeweed Antiviral Protein Isoform S1 (PAP-S1) and of Ricin-A-Chain/PAP-S1 novel fusion protein (RTA/PAP-S1) in Escherichia coli and their comparative inhibition of protein synthesis in vitro

doi: 10.1101/137919

Figure Lengend Snippet: Protein Purification. a) Stained gel of RTA/PAP-S1R68G from third wash (sample W) and from pooled eluted fraction (sample E) at 60kDa (red arrow) and of PAP-S1R68G at 30kDa (green arrow), all from native environment, after buffer exchange. The purity of sample W was 65%, of sample E 55%, and of PAP-S1R68G 60%. b) Close up and Western Blot of sample W and E. A double band is present and may be due to degradation of the protein or as a result of an unwanted recombination by E. coli cells.

Article Snippet: The E. coli pT7 expression vector and E. coli strains BL21(DE3) were purchased from Proteogenix.

Techniques: Protein Purification, Staining, Buffer Exchange, Western Blot